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One of the most discussed and sometimes confusing subjects on forums is the staging and grading of Neuroendocrine Neoplasms (NENs). Mixing them up is a common error and so it’s important to understand the difference despite the apparent complexity.
Stage vs Grade
In the most basic of terms, stage is the spread or extent of cancer and grade is the aggressiveness of cancer. They are totally different things and an understanding of both is important as they are critical to predicting outcomes (to a certain extent) and guiding therapy. There is no correlation between the two, you can have the lowest grade with the highest stage (actually very common with NETs). Remember S for Stage (Spread), G for Grade (Growing fast or slow)
The stage is essentially worked out via scans and biopsy, particularly the size of the primary tumour and the depth of any invasion into nearby tissue/organs.
The grade is worked out via biopsy only.
As patients, we deal with many medical specialists during diagnosis and subsequent treatment. However, we rarely meet the pathologist who plays a critical role in the outcome. Precise diagnosis is what drives patient decisions and treatment. If the pathology is wrong, everything that follows could be incorrect as well. It’s a very important area.
If I was to make a list of questions for my specialist/Oncologist at diagnosis, it would include “What is the stage and grade plus if I was in Grade 3, I would also want to know the ‘differentiation’ of my cancer”.
Failure to make this clear in patient groups is going to cause enormous confusion for those posting and those reading.
Words are very important in NENs
To enable me to synchronise with the documented guidance, I’m going to use the following WHO 2019 approved terms in this post and then provide an update of the key WHO 2019 changes below.
- Neuroendocrine Neoplasm (NEN) – all types of Neuroendocrine tumour of whatever grade (please note Neoplasm is another word for tumour)
- Neuroendocrine Tumour (NET) – all well-differentiated tumours, Grade 1,2 or 3 (an explanation of differentiation will be provided below)
- Neuroendocrine Carcinoma (NEC) – all poorly differentiated tumours. Neuroendocrine Carcinomas are by definition Grade 3.
NOTE: The Thoracic WHO classification committee have decided not to implement a Grade 3 well differentiated Lung NET. Consequently, the above diagram does not apply to Lung or Thoracic NETs in respect Grade 3 well differentiated NETs. Read more here.
Grading and Ki67/Mitotic Count
Grade is the aggressiveness of cancer – basically, how fast it’s growing. Neuroendocrine Neoplasms (NENs) are divided into 3 Grades depending on what range the Ki-67% measurement number lands in. With NENs, the differentiation (well or poorly) is very important when dealing with grade 3 as specialists now regard a well-differentiated tumour (i.e. a NET) with a Ki67 of less than 55% as important for outcomes and therapy decisions.
So, what is KI-67? It’s a nuclear protein associated with and may be necessary for cellular proliferation. It’s, therefore, an excellent marker to determine the growth fraction of a given cell population. The fraction of Ki-67-positive tumour cells (the Ki-67 labelling index) is often correlated with the clinical course of cancer. Pathologists normally need to count about a thousand cells to determine the percentage of cells that are Ki67 positive – thus why you see Ki67 expressed as a percentage. Zero percent is the lowest, 100% is the highest. Often, they add greater or less than signs depending on the sample involved, i.e. >5% or <5%. There are other measurement systems in place, mainly Mitotic Count which tends to be more frequently used for Lung and Thymic NENs. Even in cases of small biopsies, the Ki-67 index can usually be determined but Mitotic rate counting requires a moderate amount of tumour tissue (at least 50 HPFs or 10 mm) and that may not be feasible for small biopsies. The Mitotic Count method may be preferred or used instead or in addition to Ki-67 for certain Lung and Thymic NET scenarios as it is said to be more helpful in distinguishing atypical from typical Lung and Thymic NETs, and for small and large cell lung Neuroendocrine Carcinomas (NEC).
Please note there are major differences in how Pheochromocytomas and Paragangliomas are graded and staged, see below.
Why is differentiation important?
To fully understand grading, you also need to understand the concept of ‘differentiation’. In the most basic of terms, ‘differentiation’ refers to the extent to which the cancerous cells resemble their non-cancerous counterparts. This is an important point for high grade NENs because the differentiation of a high grade NEN could have an impact on both prognostics and treatment regimes.
Although there are 3 grades of NEN, there are two classifications of differentiation, and these become really important and only applicable at Grade 3.
Some of you may have heard the term ‘moderately differentiated’ which tended to align with an intermediate grade or Grade 2. However, please note that the term moderately differentiated as a classification was phased out in 2010 by WHO reducing from 3 differentiation levels to 2. Grade 2 is also defined as well-differentiated but based on different proliferative rate (see table).
Grading – Key WHO 2019 Changes (GEPNENs)
WHO Classifications of Cancer are published in something known in the medical world as “The Blue Book”. It’s important to understand the source of how all types of cancer (including Neuroendocrine) are named and graded, including any associated terms. This mainly comes from a World Health Organisation agency known as the International Agency for Research on Cancer (IARC). The “Blue Book” is in fact several books. Each book covers an anatomical system with the human body and Neuroendocrine Neoplasms are covered within each particular system – this is a topical point for Neuroendocrine Neoplasms given that they can appear anywhere in the body and often this causes confusion when describing cancers (e.g. we know the issues behind Pancreatic Cancer for example). There is a proposal for a single “Neuroendocrine” blue book but that has yet to come to fruition. However, the latest publication is “Digestive System” which covers most Neuroendocrine types so it’s a very useful update, and as you will see a milestone in resolving past nomenclature issues with our disease. The work behind the 2019 Digestive System follows on from the excellent work in the 2017 Endocrine update where the significant change from 2 to 3 grades of well-differentiated pancreatic NETs was a significant change. In fact, the 2017 pancreatic neuroendocrine update has been copied and pasted right into the pancreatic section of the 2019 digestive system book, effectively delivering a blue book that contains the vast majority of Gastroenteropancreatic Neuroendocrine Neoplasms (GEPNENs) including those in the hepatopancreatobiliary organs (pancreas/bile duct/liver/gallbladder). Important to also point out that a very similar structure was presented in the 2017 Endocrine Organ blue book which included (but was not limited to) Neuroendocrine related tumours of the Pituitary, Thyroid, Parathyroid, Pheochromocytoma, Paraganglioma, and their associated hereditary syndromes. ENETS and many other regions adopted this for all types of Neuroendocrine Cancer (as a result of a proposal to do so), so the 2019 Digestive System is really a rubber-stamping of this change, but it also confirms one very important difference – the word “carcinoid” has been removed from the vocabulary, not before time and something I’ve been quite vocal about since 2015. Unfortunately many doctors and healthcare establishments still remain out of date (2023!).
So, what about other areas not included in GEPNENs? Please note there are still loose ends in some of the blue books, mostly Thoracic (Lung/Thymus). Hopefully, these blue books will be updated in the same way as the others for Neuroendocrine Neoplasms (or will be subsumed into the proposed Neuroendocrine blue book). But in the meantime, experts are proposing the nomenclature above be extended in advance of the blue book updates – this is covered in Neuroendocrine neoplasm update: toward universal nomenclature, © 2020 Society for Endocrinology 2020, Guido Rindi and Frediano Inzani. What this effectively means is that the term “carcinoid” is effectively now totally defunct other than the loose ends of Carcinoid Syndrome, Carcinoid Heart Disease and Carcinoid Crisis (problems I could resolve in 10 minutes if they would listen to me). In fact, one well known pathologist has been campaigning for the removal of the terms ‘carcinoid‘ since 2007
I’ll summarise the key changes from 2019 below for your convenience, but I need to cite the article here:
Iris D Nagtegaal, Robert D Odze, David Klimstra, Valerie Paradis, Massimo Rugge, Peter Schirmacher, Kay M Washington, Fatima Carneiro, Ian A Cree, for the WHO Classification of Tumours Editorial Board. First published: 21 August 2019 – https://doi.org/10.1111/his.13975
- All well-differentiated GEPNETs now include Grade 3 alongside Grades 1 and 2.
- All poorly differentiated GEPNENs are now Neuroendocrine Carcinomas and are by default Grade 3 (the inference in the guidance is that there is no need to use the term high grade or Grade 3 in reference to Neuroendocrine Carcinomas as they have those attributes by default).
- Neuroendocrine Neoplasm is now the formal umbrella term for the grouping of well and poorly differentiated tumours.
- The term ‘carcinoid’ is no longer used in nomenclature for Neuroendocrine Neoplasms within the Digestive System blue book.
- Pancreatic Neuroendocrine Neoplasms will now appear in the Digestive System blue book, previously they were listed in the Endocrine Organ book.
- The molecular differences of well and poorly differentiated tumours have been added to assist pathologists in identifying them, e.g. Mutations in MEN1, DAXX and ATRX are entity defining for well differentiated tumours while Neuroendocrine Carcinomas (poorly differentiated) usually have TP53 or RB1 mutations.
- Poorly differentiated tumours are divided into Small Cell type (SCNEC) and Large Cell type (LCNEC).
- Last year the term “Goblet Cell Carcinoid” was changed to “Goblet Cell Carcinoma”. However, this type of appendiceal neoplasm has effectively been removed from the Neuroendocrine family as it’s now recognised to have a minor neuroendocrine component. This cancer type is now called Goblet Cell Adenocarcinoma of the Appendix.
- As per the previous content of this article, mixed tumours were renamed from Mixed AdenoNeuroendocrine Carcinomas (MANEC) are now named Mixed Neuroendocrine-Non-Neuroendocrine Neoplasms (MiNENs) – clearly they now have variable grades, variable mitotic rate/Ki67 and each component can be graded separately.
Grading – Key WHO 2021 Changes – Lung Neuroendocrine Neoplasms
Following the publication of the WHO 5th Edition of Thoracic (Lung/Thymic) Neuroendocrine Neoplasms in 2021, a new section will be added here – click here to see the differences
Previously, Pheochromocytoma/ Paraganglioma (PHEO/PGL) did not have an official grading regime, i.e. they were just benign or malignant. However, since 2017 and as reflected in the fourth edition WHO classification of Endocrine Systems, on the basis that all PHEO/PGL have some metastatic potential and have their own grading system, there are different classification systems for Pheochromocytoma and Paraganglioma These grades are based on molecular profiles (the future way for many cancers and the first time for a NET). They have a system of grading called GAPP (grading of adrenal pheochromocytoma and paraganglioma) and they are clustered into the metastatic risk (this is the beauty of molecular methods). So, they are different than regular NETs and they now have 3 differentiations, loosely related to grade. This change is complex and is covered in depth here.
Misc Grading Issues
The proliferative rate can be diverse in NENs, so sampling issues can limit the accuracy of grading. More substantial samples of tumour are therefore preferable for grading thus why the Ki-67 index is preferred for biopsies where large amounts of tissue may not be available. The distinction of low-grade from intermediate grade can be challenging when using small samples. A couple of interesting observations about NET grading which I spotted during my research and ‘forum watching’. You can have multiple primary tumours, and these might have different Ki-67 scores. Additionally, on larger tumours, Ki-67 scores can be different on different parts of the tumour. And something I know from my own experience; secondary tumours can have different Ki-67 scores than primary – even a different grade. In my own case, my liver secondary tumours were graded higher than my primary which according to my surgeon is in keeping with a clone of the disease having become more aggressive over time. Royal Free Hospital NET Centre indicates a person’s grade should be taken from the highest biopsy grade taken. This is a fairly complex area but a recent study published by the US National Institute of Health and many anecdotal comments made by NET specialists indicates that is a fairly common scenario.
Staging is the extent or spread of disease. Most types of cancer have 4 stages, numbered from 1 to 4 indicating a rising spread as the number is bigger. Often doctors write the stage down in Roman numerals, perhaps this is to stop any confusion between standard numbers used for Grades? So you may see stages written as I, II, III and IV. In addition to this standard method, there is also an agreed model known as TNM (Primary Tumour, Regional Node, Distant Metastasis) which is essentially a more detailed staging definition when combined with the Stage 1-4 model. Please note with TNM models, there could be different stage descriptions depending on the location of the primary tumour and similarly different TNM models for different tumour locations.
There are two main staging systems in use, AJCC and ENETs.
The following example shows the stage descriptions for a NET of the small intestine. Other parts of the anatomy are similar but worded accordingly for that primary location. Please don’t assume this would apply to your situation:
Stage I tumour is less than 1 cm in size and has not spread to the lymph nodes or other parts of the body.
Stage II tumour is greater than 1 cm in size and has started to spread beyond the original location but has not spread to the lymph nodes or other parts of the body.
Stage III is any size tumour that has spread to nearby areas of the body and also to at least one lymph node.
Stage IV is any size tumour that has spread to one or more lymph nodes and has also spread to other, more distant areas of the body (such as the liver).
It’s also worth pointing out that Stage IV does not necessarily mean your cancer is more dangerous than other cancers of lesser stages. This is an important point for NETs where Stage 4 can be matched up with a low-grade tumour i.e. Stage 4 for lower-grade NETs is very often not the ‘red flag’ it is for other more aggressive cancers. For example, doctors may surgically remove a Stage IV NET, while surgery might not help a patient with cancer of a higher grade at such a late stage. In NETs, grade can be the differentiator not stage.
- Sometimes doctors use the letters to further divide the number categories – for example, stage 2A or stage 3B. This is normally to clarify or provide more detail of the primary tumour size/invasion in conjunction with the TNM model.
- You may also see something called Stage 0 which is for ‘Carcinoma in situ’. It means that there is a group of abnormal cells in an area of the body. However, the number of abnormal cells is too small to form a tumour and may, therefore, be currently classed as benign. The World Health Organisation (WHO) system does not appear to recognise Stage 0 for NETs.
An example model for TNM staging is below but please note all types of NEN have their own specific TNM staging criteria (ask Ronny for yours).
i.e. a small intestine NET based on the current AJCC system (USA)
Primary Tumor (T)
- TX: primary tumor cannot be assessed
- T0: no evidence of primary tumor
- T1: invades lamina propria or submucosa and ≤ 1 cm in size
- T2: invades muscularis propria or > 1 cm in size
- T3: invades through the muscularis propria into subserosal tissue without penetration of overlying serosa
- T4: invades visceral peritoneum (serosa) or other organs or adjacent structures
T1, T2, T3 and T4 is a measure of the size of, and/or invasion/penetration by, the primary tumour and the wording varies between different NET sites. e.g. for a small intestinal NET:
For any T add (m) for multiple tumours
Regional Lymph Nodes (N) (again e.g. small intestine NET)
- NX: regional lymph nodes cannot be assessed
- N0: no regional lymph node metastasis
- N1: regional lymph node metastasis in < 12 nodes
- N2: large mesenteric masses (> 2 cm) or extensive nodal deposits (≥ 12), especially those that encase the superior mesenteric vessels
Distant Metastasis (M) (again e.g. small intestine NET)
- M0: no distant metastasis
- M1: distant metastasis
- M1a: metastasis confined to liver
- M1b: metastasis in at least one extrahepatic site (e.g., lung, ovary, nonregional lymph node, peritoneum, bone)
- M1c: both hepatic and extrahepatic metastasis
You may occasionally see TNM staging prefixed by lowercase letters. The most commonly used prefix is ‘p’ simply meaning the grading has been confirmed by pathology. e.g. pT4 N1 M1
Stage grouping – using TNM together works out the grade (again, e.g. small intestine NET)
- Stage I: T1 N0 M0
- Stage II: T2-3 N0 M0
- Stage III:T4 N0 M0 or any T N1-2 M0
- Stage IV: any T any N M1
Staging for Pheochromocytoma/Paraganglioma
This may differ between regions, but I attach the US version here – they are not normally too different. Read here.
Additional areas of understanding:
MANEC vs MiNEN
Added for completeness. This mixed and rare neoplasm type has traditionally been related to NEC but in 2017 the nomenclature change to a new term was necessary to reflect the fact that some of the tumours involved were not carcinomas or adenocarcinomas but rather were well-differentiated tumours or even adenomas (i.e. benign). Previously known as Mixed AdenoNeuroendocrine Carcinoma (MANEC), they were renamed to Mixed Neuroendocrine Non-Neuroendocrine Neoplasms (MiNEN).
MiNEN are neoplasms with two distinct neuroendocrine and non-neuroendocrine cell populations. They can be morphologically classified into three entities: collision, composite, and amphicrine MiNEN. Currently, both components composing a MiNEN must represent at least 30% of the whole tumour. Diagnosis of MiNEN is usually facilitated by the presence of at least one well-differentiated component which may be the Neuroendocrine or Non-Neuroendocrine component. However, the two components may be difficult to identify with conventional morphological techniques, particularly when they are poorly differentiated, and their identification may require additional immunohistochemical techniques. MiNEN usually originates from organs that contain neuroendocrine cells and in which “classical” NENs are known to develop, such as the pancreas, appendix, colon, and to a lesser degree small intestine. Other locations in my source document include Oesophagus, Stomach, Biliary Tract and Gallbladder, Duodenum, and Ampulla of Vater and Rectum.
NEC vs NET
Having researched widely, I believe there are 8 key differences between NET and NEC:
- Grade – NETs can be Grade 1, 2 or 3. There is no requirement to use Grade 3 when talking about Neuroendocrine Carcinoma which by default is poorly differentiated high grade.
- Differentiation – all NETs are well differentiated, NECs are poorly differentiated. Important difference at Grade 3 NET.
- Aggressiveness – Most NETs tend to be indolent or slow growing while NECs tend to be aggressive and faster growing. However, Ki67 and/or mitotic count is an aggressiveness measurement tool. Genetic profiles can also be a guide but this is beyond the purposes of this article but may be explored in subsequent parts. It follows that NECs normally have a worse prognosis in comparison to NETs.
- Hormone Secretion – NETs can produce peptide hormones that may be associated with hormonal syndromes. NECs usually fail to express hormones or produce hormonal syndromes.
- Somatostatin Receptors – A NET is much more likely to express somatostatin receptors which can influence treatments such as somatostatin analogues and peptide receptor radiotherapy (PRRT)
- Hereditary Syndromes – NETs are much more likely to be associated with hereditary syndromes such as Multiple Endocrine Neoplasia (MEN).
- Platinum Based Chemotherapy – NETs are less likely to show a good response to platinum based chemotherapy which can often be the first line treatment for NEC.
- Primary Locations – can be vastly different in terms of commonality and therefore provide clues to investigators. Common locations for NEC include: Lung, Esophagus, Colon, Urogential Organs and Skin – with the exception of Lung, these are very rare locations in NETs. Conversely, rare/very rare locations for NEC but common in NET include Rectal, Small Intestine, Appendix, Stomach, Pancreas.
A complex area and I hope I have condensed it sufficiently for you to understand enough for your purposes. Despite looking very scientific, it is not an exact science. There are many variables as there always are with Neuroendocrine disease. NENs can be very challenging for a pathologist even an experienced one who may not have encountered NENs before. However, it is an extremely important part of initial diagnosis and also when needed during surveillance. It is a vital tool used by Multidisciplinary Teams (MDT) in treatment plans and for prognostic purposes. If you need to learn further, I recommend this document:
If you are interested in this subject and have one hour to spare, there is a great video here from LACNETS worth watching.
1. WHO Classification of Tumours of Endocrine Organs, latest edition.
2. WHO Classification of Tumours of Digestive System, latest edition.
3. WHO Classification of Tumours of Thoracic System, latest edition.
4. The UICC TNM classification (the internationally accepted standard for cancer staging).
5. AJCC 8th Edition Cancer Staging.
5. ENETS Staging.
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